2-furoyl coenzyme A dehydrogenase, 2-furoyl coenzyme A hydroxylase, dehydrogenase, 2-furoyl coenzyme A, furoyl-CoA hydroxylase
EC Number
1.3.99.8
CAS Registry Number
9068-18-2
Comments
A copper protein. The oxygen atom of the -OH produced is derived from water, not O2; the actual oxidative step is probably dehydrogenation of a hydrated form -CHOH-CH2- to -C(OH)=CH-, which tautomerizes non-enzymically to -CO-CH2-, giving (5-oxo-4,5-dihydro-2-furoyl)-CoA. Methylene blue, nitro blue, tetrazolium and a membrane fraction from Pseudomonas putida can act as acceptors.
