| Synonyms |
2′-phosphotransferase, AtPt, HeLa cell 2′-phosphotransferase, human TRPT1, KptA, mTPT1, NAD+-dependent 2′-phosphotransferase, NAD-dependent RNA 2′-phosphotransferase, RNA 2′-phosphotransferase, Tpt1, Tpt1p, tRNA 2′-phosphotransferase, Trpt1, yeast 2′-phosphotransferase |
| Comments |
Catalyses the final step of tRNA splicing in the yeast?Saccharomyces cerevisiae?[2]. The reaction takes place in two steps: in the first step, the 2′-phosphate on the RNA substrate is ADP-ribosylated, causing the relase of nicotinamide and the formation of the reaction intermediate, ADP-ribosylated tRNA [6]. In the second step, dephosphorylated (mature) tRNA is formed along with ADP ribose 1”,2”-cyclic phosphate. Highly specific for oligonucleotide substrates bearing an internal 2′-phosphate. Oligonucleotides with only a terminal 5′- or 3′-phosphate are not substrates [1]. |
