23S rRNA (adenine2503-C2)-methyltransferase EC#: 2.1.1.192; ChemWhat Code: 1376415

Product Name 23S rRNA (adenine2503-C2)-methyltransferase
Example Structure Example Structure of 23S rRNA (adenine2503-C2)-methyltransferase EC#: 2.1.1.192
Synonyms Cfr, methyltransferase YfgB/RlmN, radical AdoMet rRNA methyltransferase, radical S-adenosyl-L-methionine enzyme, radical SAM enzyme, RlmN, RlmN methyltransferase, YfgB, YfgB/RlmN
EC Number 2.1.1.192
CAS Registry Number
Comments Contains an [4Fe-4S] cluster [2]. This enzyme is a member of the ‘AdoMet radical’ (radical SAM) family. S-adenosyl-L-methionine acts as both a radical generator and as the source of the appended methyl group. RlmN first transfers an CH2 group to a conserved cysteine (Cys355 in Escherichia coli) [6], the generated radical from a second S-adenosyl-L-methionine then attacks the methyl group, exctracting a hydrogen. The formed radical forms a covalent intermediate with the adenine group of the tRNA [9]. RlmN is an endogenous enzyme used by the cell to refine functions of the ribosome in protein synthesis [2]. The enzyme methylates adenosine by a radical mechanism with CH2 from the S-adenosyl-L-methionine and retention of the hydrogen at C-2 of adenosine2503 of 23S rRNA. It will also methylate 8-methyladenosine2503 of 23S rRNA. cf. EC 2.1.1.224 [23S rRNA (adenine2503-C8)-methyltransferase].
Cofactor iron-sulfur
History
Reactions (1) 2 S-adenosyl-L-methionine + adenine(2503) in 23S rRNA + 2 reduced [2Fe-2S] ferredoxin = S-adenosyl-L-homocysteine + L-methionine + 5′-deoxyadenosine + 2-methyladenine(2503) in 23S rRNA + 2 oxidized [2Fe- 2S] ferredoxin. (2) 2 S-adenosyl-L-methionine + adenine(37) in tRNA + 2 reduced [2Fe-2S] ferredoxin = S-adenosyl-L-homocysteine + L-methionine + 5′-deoxyadenosine + 2-methyladenine(37) in tRNA + 2 oxidized [2Fe-2S] ferredoxin.

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