The enzyme, characterized from the bacterium?Nocardioides?sp. JS1661, is involved in the degradation of 2,4-dinitroanisole. Unlike other known?O-demethylases, such as EC?1.14.99.15, 4-methoxybenzoate monooxygenase (O-demethylating), or EC?1.14.11.32, codeine 3-O-demethylase, it does not require oxygen or electron donors, and produces methanol rather than formaldehyde.