| Product Name |
25/26-hydroxycholesterol 7alpha-hydroxylase |
| Example Structure |
 |
| Synonyms |
25-hydroxycholesterol 7alpha-hydroxylase, 25-hydroxycholesterol 7alpha-monooxygenase, 25-hydroxycholesterol-7alpha-hydroxylase, cholesterol 25-hydroxylase, Cyp39a1, CYP7B, CYP7B1, CYP7B1 oxysterol 7alpha-hydroxylase, CYPB1, cytochome P450 7B1, cytochrome P-450 oxysterol 7-alpha-hydroxylase, oxysterol 7 alpha-hydroxylase, oxysterol 7alpha-hydroxylase, oxysterol-7alpha-hydroxylase, Steroid hydroxylase |
| EC Number |
1.14.14.29 |
| CAS Registry Number |
|
| Comments |
A P450 (heme-thiolate) protein. Unlike EC 1.14.14.26, 24-hydroxycholesterol 7¦Á-monooxygenase, which is specific for its oxysterol substrate, this enzyme can also metabolize the oxysterols 24,25-epoxycholesterol, 22-hydroxycholesterol and 24-hydroxycholesterol, but to a lesser extent [2]. The direct electron donor to the enzyme is EC 1.6.2.4, NADPH¡ªhemoprotein reductase. |
| Cofactor |
heme-thiolate |
| History |
|
| Reactions |
(1) Cholest-5-ene-3-beta,25-diol + [reduced NADPH–hemoprotein reductase] + O(2) = cholest-5-ene-3-beta,7-alpha,25-triol + [oxidized NADPH- -hemoprotein reductase] + H(2)O. (2) (25R)-cholest-5-ene-3-beta,26-diol + [reduced NADPH–hemoprotein reductase] + O(2) = (25R)-cholest-5-ene-3-beta,7-alpha,26-triol + [oxidized NADPH–hemoprotein reductase] + H(2)O. |
