| Synonyms |
6-deoxyerythronolide B, 6-deoxyerythronolide B synthase, DEBS, DEBS1, DEBS2, DEBS3, deoxyerythronolide B synthase, deoxyerythronolide B synthase 1, deoxyerythronolide B synthase 2, deoxyerythronolide B synthase 3, erythromycin polyketide synthase, erythronolide condensing enzyme, erythronolide synthase, PKS, polyketide synthase, synthase, erythronolide |
| Comments |
The product, 6-deoxyerythronolide B, contains a 14-membered lactone ring and is an intermediate in the biosynthesis of erythromycin antibiotics. Biosynthesis of 6-deoxyerythronolide B requires 28 active sites that are precisely arranged along three large polypeptides, denoted DEBS1, -2 and -3 [6]. The polyketide product is synthesized by the processive action of a loading didomain, six extension modules and a terminal thioesterase domain [5]. Each extension module contains a minimum of a ketosynthase (KS), an acyltransferase (AT) and an acyl-carrier protein (ACP). The KS domain both accepts the growing polyketide chain from the previous module and catalyses the subsequent decarboxylative condensation between this substrate and an ACP-bound methylmalonyl extender unit, introduce by the AT domain. This combined effort gives rise to a new polyketide intermediate that has been extended by two carbon atoms [5]. |
