The enzyme produces the F0?precursor of the redox cofactor coenzyme F420, which is found in methanogens and in various actinobacteria. F0?is also produced by some cyanobacteria and eukaryotes. The enzyme, which forms a complex with EC?2.5.1.147, 5-amino-6-(D-ribitylamino)uracil¡ªtyrosine 4-methylphenol transferase, is a radical SAM enzyme that uses the 5′-deoxyadenosyl radical to catalyse the condensation reaction.