Recombinant Canine Granulocyte-Macrophage Colony Stimulating Factor (rCaGM-CSF, P48749, 403923) CAS#: 132-03-1816; ChemWhat Code: 1404352

Official Full Name Recombinant Canine Granulocyte-Macrophage Colony Stimulating Factor (rCaGM-CSF)
Squence Squence of Recombinant Canine Granulocyte-Macrophage Colony Stimulating Factor
Amino Acid Sequence APTRSPTLVT RPSQHVDAIQ EALSLLNNSN DVTAVMNKAV KVVSEVFDPE GPTCLETRLQ LYKEGLQGSL TSLKNPLTMM ANHYKQHCPP TPESPCATQN INFKSFKENL KDFLFNIPFD CWKPVKK
Synonyms Granulocyte/Macrophage Colony-Stimulating Factor, CSF-2, MGI-1GM, Pluripoietin-α
Accession Number P48749
GeneID 403923
Summary
Source Escherichia coli.
Molecular Weight Approximately 14.2 kDa, a single non-glycosylated polypeptide chain containing 127 amino acids.
Biological Activity Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 5 ng/ml, corresponding to a specific activity of > 2.0 × 105 IU/mg.
Appearance Sterile filtered white lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Endotoxin Less than 1 EU/ug of rCaGM-CSF as determined by LAL method.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
References 1. Wang JM, Chen ZG, Colotta F, et al. 1988. Behring Inst Mitt: 270-3.2. 1989. N Engl J Med, 320: 253-4.3. Nissen-Druey C. 1989. Nouv Rev Fr Hematol, 31: 99-101.4. Eager RandNemunaitis J. 2005. Mol Ther, 12: 18-27.5. Tran T, Fernandes DJ, Schuliga M, et al. 2005. Br J Pharmacol, 145: 123-31.
SDS-PAGE SDS-PAGE of Recombinant Canine Granulocyte-Macrophage Colony Stimulating Factor
Safety Data Sheet (SDS) Download Click to download
Technical Data Sheet (TDS) Download Click to download

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