Recombinant Flagellin, His (rFlagellin,His, P06179, 1253480) CAS#: 661-02-1816; ChemWhat Code: 1404118

Official Full Name Recombinant Flagellin, His (rFlagellin,His)
Squence Squence of Recombinant Flagellin, His
Amino Acid Sequence MAQVINTNSL SLLTQNNLNK SQSALGTAIE RLSSGLRINS AKDDAAGQAI ANRFTANIKG LTQASRNAND GISIAQTTEG ALNEINNNLQ RVRELAVQSA NSTNSQSDLD SIQAEITQRL NEIDRVSGQT QFNGVKVLAQ DNTLTIQVGA NDGETIDIDL KQINSQTLGL DTLNVQQKYK VSDTAATVTG YADTTIALDN STFKASATGL GGTDQKIDGD LKFDDTTGKY YAKVTVTGGT GKDGYYEVSV DKTNGEVTLA GGATSPLTGG LPATATEDVK NVQVANADLT EAKAALTAAG VTGTASVVKM SYTDNNGKTI DGGLAVKVGD DYYSATQNKD GSISINTTKY TADDGTSKTA LNKLGGADGK TEVVSIGGKT YAASKAEGHN FKAQPDLAEA AATTTENPLQ KIDAALAQVD TLRSDLGAVQ NRFNSAITNL GNTVNNLTSA RSRIEDSDYA TEVSNMSRAQ ILQQAGTSVL AQANQVPQNV LSLLRLEHHH HHH
Synonyms
Accession Number P06179
GeneID 1253480
Summary
Source Escherichia coli.
Molecular Weight Approximately 52.7 kDa, a single non-glycosylated polypeptide chain containing 503 amino acids, with Leu, Glu and 6 × His at C-terminus.
Biological Activity No data available
Appearance Sterile filtered white lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Endotoxin Less than 1 EU/ug of rFlagellin, His as determined by LAL method.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
References 1. Fedorov OVandKostyukova AS. 1984. FEBS Lett, 171: 145-8.2. Belas RandFlaherty D. 1994. Gene, 148: 33-41.3. Givaudan A, Lanois A, Boemare N. 1996. Gene, 183: 243-53.4. Meindl T, Boller T, Felix G. 2000. Plant Cell, 12: 1783-94.5. Hayashi F, Smith KD, Ozinsky A, et al. 2001. Nature, 410: 1099-103.
SDS-PAGE SDS-PAGE of Recombinant Flagellin, His
Safety Data Sheet (SDS) Download Click to download
Technical Data Sheet (TDS) Download Click to download

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