| Official Full Name |
Recombinant Human Angiostatin (rHuAngiostatin) |
| Squence |
 |
| Amino Acid Sequence |
VYLSECKTGN GKNYRGTMSK TKNGITCQKW SSTSPHRPRF SPATHPSEGL EENYCRNPDN DPQGPWCYTT DPEKRYDYCD ILECEEECMH CSGENYDGKI SKTMSGLECQ AWDSQSPHAH GYIPSKFPNK NLKKNYCRNP DRELRPWCFT TDPNKRWELC DIPRCTTPPP SSGPTYQCLK GTGENYRGNV AVTVSGHTCQ HWSAQTPHTH NRTPENFPCK NLDENYCRNP DGKRAPWCHT TNSQVRWEYC KIPSCDSSP |
| Synonyms |
|
| Accession Number |
P00747 |
| GeneID |
5340 |
| Summary |
|
| Source |
Escherichia coli. |
| Molecular Weight |
Approximately 29.7 KDa, a single non-glycosylated polypeptide chain containing 259 amino acids. |
| Biological Activity |
Fully biologically active when compared to standard. The specific activity determined by an assay on anti-proliferation and anti-migration using endothelial cells in vitro and anti-angiogenesis in vivo is 5.5 × 105 IU/mg. |
| Appearance |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation |
Lyophilized from a 0.2 um filtered concentrated solution in 20 mM NaAc, pH 5.5, 4 % mannitol. |
| Endotoxin |
Less than 1 EU/ug of rHuAngiostatin as determined by LAL method. |
| Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Stability and Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| References |
1. Takahashi S, Shinya T, Sugiyama A. 2010. J Pharmacol Sci, 112: 432-7.2. Frank KFandMuller-Ehmsen J. 2009. Heart, 95: 269-70.3. Perri SR, Annabi B, Galipeau J. 2007. FASEB J, 21: 3928-36.4. Kim KS, Kim HS, Park JS, et al. 2004. Cancer Gene Ther, 11: 441-9.5. Wahl ML, Owen CS, Grant DS. 2002. Endothelium, 9: 205-16. |
| SDS-PAGE |
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| Safety Data Sheet (SDS) Download |
Click to download |
| Technical Data Sheet (TDS) Download |
Click to download |
