Recombinant Human Beta-defensin 3 (rHuBD-3, P81534, 55894) CAS#: 109-03-1816

Official Full Name Recombinant Human Beta-defensin 3 (rHuBD-3)
Squence Squence of Recombinant Human Beta-defensin 3
Amino Acid Sequence GIINTLQKYY CRVRGGRCAV LSCLPKEEQI GKCSTRGRKC CRRKK
Synonyms Defensin beta3
Accession Number P81534
GeneID 55894
Summary
Source Escherichia coli.
Molecular Weight Approximately 5.2 kDa, a single non-glycosylated polypeptide chain containing 45 amino acids.
Biological Activity Fully biologically active when compared to standard. The ED50 as determined by anti-microbial activity against E.coli. is less than 30 ug/ml, corresponding to a specific activity of > 33.3 IU/mg.
Appearance Sterile filtered white lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 130 mM NaCl.
Endotoxin Less than 1 EU/ug of rHuBD-3 as determined by LAL method.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
References 1. Lioi AB, Reyes Rodriguez AL, Funderburg NT, et al. 2012. J Leukoc Biol, 92: 1083-91.2. Wang K, Wang JH, Baskaran H, et al. 2012. Head Neck Oncol, 4: 41.3. Lee JK, Park YJ, Kum KY, et al. 2012. Int Endod J,
SDS-PAGE SDS-PAGE of Recombinant Human Beta-defensin 3
Safety Data Sheet (SDS) Download Click to download
Technical Data Sheet (TDS) Download Click to download

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