| Official Full Name |
Recombinant Human Ciliary Neurotrophic Factor (rHuCNTF) |
| Squence |
 |
| Amino Acid Sequence |
MAFTEHSPLT PHRRDLCSRS IWLARKIRSD LTALTESYVK HQGLNKNINL DSADGMPVAS TDQWSELTEA ERLQENLQAY RTFHVLLARL LEDQQVHFTP TEGDFHQAIH TLLLQVAAFA YQIEELMILL EYKIPRNEAD GMPINVGDGG LFEKKLWGLK VLQELSQWTV RSIHDLRFIS SHQTGIPARG SHYIANNKKM |
| Synonyms |
CNTF |
| Accession Number |
P26441 |
| GeneID |
1270 |
| Summary |
|
| Source |
Escherichia coli. |
| Molecular Weight |
Approximately 22.9 kDa, a single non-glycosylated polypeptide chain containing 200 amino acids. |
| Biological Activity |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 150 ng/ml, corresponding to a specific activity of > 6.7 × 103 IU/mg. |
| Appearance |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation |
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4. |
| Endotoxin |
Less than 1 EU/ug of rHuCNTF as determined by LAL method. |
| Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Stability and Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| References |
1. Lam A, Fuller F, Miller J, et al. 1991. Gene. 102:271-6.2. Bazan JF. 1991. Neuron. 7:197-208.3. McGregor NE, Poulton IJ, Walker EC, et al. 2010. Calcif Tissue Int. 86:261-70.4. Lambert PD, Anderson KD, Sleeman MW, et al. 2001. Proc Natl Acad Sci U S A. 98:4652-7. |
| SDS-PAGE |
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| Safety Data Sheet (SDS) Download |
Click to download |
| Technical Data Sheet (TDS) Download |
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