Official Full Name |
Recombinant Human Epithelial Neutrophil Activating Peptide-78, 8-78 a.a./CXCL5 (rHuENA-78,8-78a.a./CXCL5) |
Squence |
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Amino Acid Sequence |
LRELRCVCLQ TTQGVHPKMI SNLQVFAIGP QCSKVEVVAS LKNGKEICLD PEAPFLKKVI QKILDGGNKE N |
Synonyms |
CXCL5, Small-inducible cytokine B5 |
Accession Number |
P42830 |
GeneID |
6374 |
Summary |
|
Source |
Escherichia coli. |
Molecular Weight |
Approximately 7.8 kDa, a single non-glycosylated polypeptide chain containing 71 amino acids. |
Biological Activity |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood neutrophils is in a concentration of 5.0-10.0 ng/ml. |
Appearance |
Sterile filtered white lyophilized (freeze-dried) powder. |
Formulation |
Lyophilized from a 0.2 um filtered concentrated solution in 2 × PBS, pH 7.4. |
Endotoxin |
Less than 1 EU/ug of rHuENA-78, 8 – 78 a.a./CXCL5 as determined by LAL method. |
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
Stability and Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
References |
1. Chang MS, McNinch J, Basu R, et al. 1994. J Biol Chem. 269:25277-82.2. O’Donovan N, Galvin M, Morgan JG. 1999. Cytogenet Cell Genet. 84:39-42.3. Persson T, Monsef N, Andersson P, et al. 2003. Clin Exp Allergy. 33:531-7.4. Wuyts A, Govaerts C, Struyf S, et al. 1999. Eur J Biochem. 260:421-9. |
SDS-PAGE |
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Safety Data Sheet (SDS) Download |
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Technical Data Sheet (TDS) Download |
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