Recombinant Human Fibroblast Growth Factor 21 (rHuFGF-21, Q9NSA1, 26291) CAS#: 104-21-1816; ChemWhat Code: 1405215

Official Full Name Recombinant Human Fibroblast Growth Factor 21 (rHuFGF-21)
Squence Squence of Recombinant Human Fibroblast Growth Factor 21
Amino Acid Sequence HPIPDSSPLL QFGGQVRQRY LYTDDAQQTE AHLEIREDGT VGGAADQSPE SLLQLKALKP GVIQILGVKT SRFLCQRPDG ALYGSLHFDP EACSFRELLL EDGYNVYQSE AHGLPLHLPG NKSPHRDPAP RGPARFLPLP GLPPALPEPP GILAPQPPDV GSSDPLSMVG PSQGRSPSYA S
Synonyms
Accession Number Q9NSA1
GeneID 26291
Summary
Source
Molecular Weight Approximately 19.4 kDa, a single non-glycosylated polypeptide chain containing 181 amino acids.
Biological Activity Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ug/ml, corresponding to a specific activity of > 2.0 × 103 IU/mg in the presence of 5 ug/ml of rMuKlotho-beta and 10 ug/ml of heparin.
Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Endotoxin Less than 1 EU/ug of rHuFGF-21 as determined by LAL method.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
References
SDS-PAGE SDS-PAGE of Recombinant Human Fibroblast Growth Factor 21
Safety Data Sheet (SDS) Download Click to download
Technical Data Sheet (TDS) Download Click to download

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