Official Full Name |
Recombinant Human I-309/CCL1 (rHuI-309/CCL1) |
Squence |
 |
Amino Acid Sequence |
SKSMQVPFSR CCFSFAEQEI PLRAILCYRN TSSICSNEGL IFKLKRGKEA CALDTVGWVQ RHRKMLRHCP SKRK |
Synonyms |
Small-inducible cytokine A1, T lymphocyte-secreted protein I-309 |
Accession Number |
P22362 |
GeneID |
6346 |
Summary |
Chemokine (C-C motif) ligand 1 (CCL1) belongs to a family inflammatory cytokines and also known as chemokines. It is a small glycoprotein secreted by activated T cells with a molecular weight of approximately 8.5 kDa. CCL1 attracts monocytes, NK cells, and immature B cells and dendritic cells by interacting with a cell surface chemokine receptor called CCR8. Human CCL1 has been assumed to be a homologue of the mouse TCA3. While the two proteins share only approximately 42 % amino acid sequence identity, both chemokines contain an extra pair of cysteine residues not found in most other chemokines. |
Source |
Escherichia coli. |
Molecular Weight |
Approximately 8.6 kDa, a single, non-glycosylated polypeptide chain containing 74 amino acids. |
Biological Activity |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 10-100 ng/ml. |
Appearance |
Sterile filtered white lyophilized (freeze-dried) powder. |
Formulation |
Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl. |
Endotoxin |
Less than 1 EU/ug of rHuI-309/CCL1 as determined by LAL method. |
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
Stability and Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
References |
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SDS-PAGE |
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Safety Data Sheet (SDS) Download |
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Technical Data Sheet (TDS) Download |
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