| Official Full Name |
Recombinant Human Indian Hedgehog Cys28IleIle (rHuIHHC28II) |
| Squence |
 |
| Amino Acid Sequence |
IIGPGRVVGS RRRPPRKLVP LAYKQFSPNV PEKTLGASGR YEGKIARSSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDRLNSLAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRNKYGLL ARLAVEAGFD WVYYESKAHV HCSVKSEHSA AAKTGG |
| Synonyms |
HHG-2 |
| Accession Number |
Q14623 |
| GeneID |
3549 |
| Summary |
|
| Source |
Escherichia coli. |
| Molecular Weight |
Approximately 19.8 kDa, a single non-glycosylated polypeptide chain containing 176 amino acids. |
| Biological Activity |
Fully biologically active when compared to standard. The ED50 as determined by its ability to induce alkaline phosphatase production by C3H10T1/2(CCL-226) cells is 3.0-10 ug/ml. |
| Appearance |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation |
Lyophilized from a 0.2 um filtered concentrated solution in 1 × PBS, pH 7.4. |
| Endotoxin |
Less than 1 EU/ug of rHuIHH C28II as determined by LAL method. |
| Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Stability and Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| References |
1. Kumar S, Balczarek KA, Lai ZC. 1996. Genetics. 142:965-72.2. Marigo V, Roberts DJ, Lee SM, et al. 1995. Genomics. 28:44-51.3. Vortkamp A, Lee K, Lanske B, et al. 1996. Science. 273:613-22. |
| SDS-PAGE |
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| Safety Data Sheet (SDS) Download |
Click to download |
| Technical Data Sheet (TDS) Download |
Click to download |
