Recombinant Human Interleukin-36 gamma, 152a.a. (rHuIL-36gamma,152a.a., Q9NZH8, 56300) CAS#: 101-366-1816; ChemWhat Code: 1404726

Official Full NameRecombinant Human Interleukin-36 gamma, 152a.a. (rHuIL-36gamma,152a.a.)
SquenceSquence of Recombinant Human Interleukin-36 gamma, 152a.a.
Amino Acid SequenceSMCKPITGTI NDLNQQVWTL QGQNLVAVPR SDSVTPVTVA VITCKYPEAL EQGRGDPIYL GIQNPEMCLY CEKVGEQPTL QLKEQKIMDL YGQPEPVKPF LFYRAKTGRT STLESVAFPD WFIASSKRDQ PIILTSELGK SYNTAFELNI ND
SynonymsIL-1RP2, IL-1 epsilon, IL-1F9, IL-1H1
Accession NumberQ9NZH8
GeneID56300
SummaryInterleukin-36 (IL-36) is a pro-inflammatory cytokine which plays an important role in the pathophysiology of several diseases. IL-36alpha, IL-36β, and IL-36gamma (formerly IL-1F6, IL-1F8, and IL-1F9) are IL-1 family members that signal through the IL-1 receptor family members IL-1Rrp2 (IL-1RL2) and IL-1RAcP. IL-36gamma is secreted when transfected into 293-T cells and it could constitute part of an independent signaling system analogous to interleukin-1 alpha (IL-1A), beta (IL-1B) receptor agonist and interleukin-1 receptor type I (IL-1R1). Furthermore, IL-36gamma also can function as an agonist of NF-kappa B activation through the orphan IL-1-receptor-related protein 2. Human IL-36gamma (152a.a) shares 58 %, 59 %, 68 % and 69 % a.a. sequence identity with mouse, rat, bovine and equine IL-36gamma, respectively, and 23 – 57% a.a. sequence identity with other family members.
SourceEscherichia coli.
Molecular WeightApproximately 17.0 kDa, a single non-glycosylated polypeptide chain containing 152 amino acids.
Biological ActivityFully biologically active when compared to standard. The ED50 as determined by its ability to induce IL-8 secretion by human preadipocytes is less than 10 ng/ml, corresponding to a specific activity of > 1 × 105 IU/mg.
AppearanceSterile filtered white lyophilized (freeze-dried) powder.
FormulationLyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
EndotoxinLess than 1 EU/ug of rHuIL-36gamma, 152a.a. as determined by LAL method.
ReconstitutionWe recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.
Stability and StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
References
SDS-PAGESDS-PAGE of Recombinant Human Interleukin-36 gamma, 152a.a.
Safety Data Sheet (SDS) DownloadClick to download
Technical Data Sheet (TDS) DownloadClick to download

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