| Official Full Name |
Recombinant Human Interleukin-4 GMP (rHuIL-4) |
| Squence |
 |
| Amino Acid Sequence |
HKCDITLQEI IKTLNSLTEQ KTLCTELTVT DIFAASKNTT EKETFCRAAT VLRQFYSHHE KDTRCLGATA QQFHRHKQLI RFLKRLDRNL WGLAGLNSCP VKEANQSTLE NFLERLKTIM REKYSKCSS |
| Synonyms |
BSF-1, Binetrakin, Lymphocyte stimulatory factor 1, Pitrakinra. |
| Accession Number |
P05112 |
| GeneID |
3565 |
| Summary |
|
| Source |
Escherichia coli. |
| Molecular Weight |
Approximately 15.0 kDa, a single non-glycosylated polypeptide chain containing 129 amino acids. |
| Biological Activity |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.2 ng/ml, corresponding to a specific activity of > 5.0 × 106 IU/mg. |
| Appearance |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation |
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4. |
| Endotoxin |
Less than 0.01 EU/ug of rHuIL-4 GMP as determined by LAL method. |
| Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Stability and Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- A minimum of 12 months when stored at ≤ -20 °C as supplied. Refer to lot specific COA for the Use by Date.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| References |
1. Sokol CL, Barton GM, Farr AG, et al. 2008. Nat Immunol. 9:310-8.2. Hershey GK, Friedrich MF, Esswein LA, et al. 1997. N Engl J Med. 337:1720-5.3. Yokota T, Otsuka T, Mosmann T, et al. 1986. Proc Natl Acad Sci U S A. 83:5894-8.4. Eder A, Krafft-Czepa H, Krammer PH. 1988. Nucleic Acids Res. 16:772.5. Walter MR, Cook WJ, Zhao BG, et al. 1992. J Biol Chem. 267:20371-6. |
| SDS-PAGE |
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| Safety Data Sheet (SDS) Download |
Click to download |
| Technical Data Sheet (TDS) Download |
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