Official Full Name |
Recombinant Human Keratinocyte Growth Factor-1/FGF-7 GMP (rHuKGF-1/FGF-7) |
Squence |
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Amino Acid Sequence |
CNDMTPEQMA TNVNCSSPER HTRSYDYMEG GDIRVRRLFC RTQWYLRIDK RGKVKGTQEM KNNYNIMEIR TVAVGIVAIK GVESEFYLAM NKEGKLYAKK ECNEDCNFKE LILENHYNTY ASAKWTHNGG EMFVALNQKG IPVRGKKTKK EQKTAHFLPM AIT |
Synonyms |
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Accession Number |
P21781 |
GeneID |
2252 |
Summary |
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Source |
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Molecular Weight |
Approximately 18.9 kDa, a single, non-glycosylated polypeptide chain containing 163 amino acids. |
Biological Activity |
Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 10 ng/ml, corresponding to a specific activity of > 1.0 × 105 IU/mg. |
Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
Formulation |
Lyophilized from a 0.2 um filtered solution in 20mM PB, pH 7.4, 500 mM NaCl, 0.02 % Tween-20. |
Endotoxin |
Less than 0.01 EU/ug of rHuKGF-1/FGF-7 GMP as determined by LAL method. |
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
Stability and Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.A minimum of 12 months when stored at ≤ -20 °C as supplied. Refer to lot specific COA for the Use by Date.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution. |
References |
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SDS-PAGE |
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Safety Data Sheet (SDS) Download |
Click to download |
Technical Data Sheet (TDS) Download |
Click to download |