Recombinant Human Macrophage Inflammatory Protein-1 beta/CCL4 (rHuMIP-1beta/CCL4, P13236, 6351) CAS#: 204-04-1816

Official Full Name Recombinant Human Macrophage Inflammatory Protein-1 beta/CCL4 (rHuMIP-1beta/CCL4)
Squence Squence of Recombinant Human Macrophage Inflammatory Protein-1 beta/CCL4
Amino Acid Sequence APMGSDPPTA CCFSYTARKL PRNFVVDYYE TSSLCSQPAV VFQTKRSKQV CADPSESWVQ EYVYDLELN
Synonyms G-26 T-lymphocyte-secreted protein, HC21, LAG-1, MIP-1-beta, PAT 744, Protein H400, SIS-gamma, Small-inducible cytokine A4, ACT-2
Accession Number P13236
GeneID 6351
Summary
Source Escherichia coli.
Molecular Weight Approximately 7.8 kDa, a single non-glycosylated polypeptide chain containing 69 amino acids.
Biological Activity Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 5.0-20 ng/ml.
Appearance Sterile filtered white lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
Endotoxin Less than 1 EU/ug of rHuMIP-1β/CCL4 as determined by LAL method.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
References 1. Irving SG, Zipfel PF, Balke J, et al. 1990. Nucleic Acids Res. 18:3261-70.2. Cocchi F, DeVico AL, Garzino-Demo A, et al. 1995. Science. 270:1811-5.3. Garlisi CG, Xiao H, Tian F, et al. 1999. Eur J Immunol. 29:3210-5.4. Guan E, Wang J, Roderiquez G, et al. 2002. J Biol Chem. 277:32348-52.
SDS-PAGE SDS-PAGE of Recombinant Human Macrophage Inflammatory Protein-1 beta/CCL4
Safety Data Sheet (SDS) Download Click to download
Technical Data Sheet (TDS) Download Click to download

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