| Official Full Name |
Recombinant Human Monocyte Chemotactic Protein-4/CCL13 (rHuMCP-4/CCL13) |
| Squence |
 |
| Amino Acid Sequence |
QPDALNVPST CCFTFSSKKI SLQRLKSYVI TTSRCPQKAV IFRTKLGKEI CADPKEKWVQ NYMKHLGRKA HTLKT |
| Synonyms |
CK-beta-10, NCC-1, Small-inducible cytokine A13 |
| Accession Number |
Q99616 |
| GeneID |
6357 |
| Summary |
|
| Source |
Escherichia coli. |
| Molecular Weight |
Approximately 8.6 kDa, a single non-glycosylated polypeptide chain containing 75 amino acids. |
| Biological Activity |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration of 10-100 ng/ml. |
| Appearance |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation |
Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 130 mM NaCl. |
| Endotoxin |
Less than 1 EU/ug of rHuMCP-4/CCL13 as determined by LAL method. |
| Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Stability and Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| References |
1. Uguccioni M, Loetscher P, Forssmann U, et al. 1996. J Exp Med. 183:2379-84.2. Berkhout TA, Sarau HM, Moores K, et al. 1997. J Biol Chem. 272:16404-13.3. Garcia-Zepeda EA, Combadiere C, Rothenberg ME, et al. 1996. J Immunol. 157:5613-26. |
| SDS-PAGE |
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| Safety Data Sheet (SDS) Download |
Click to download |
| Technical Data Sheet (TDS) Download |
Click to download |
