Recombinant Human Nephroblastoma-overexpressed Gene Protein Homolog (rHuNOV, P48745, 4856) CAS#: 105-0129-1816; ChemWhat Code: 1405207

Official Full Name Recombinant Human Nephroblastoma-overexpressed Gene Protein Homolog (rHuNOV)
Squence Squence of Recombinant Human Nephroblastoma-overexpressed Gene Protein Homolog
Amino Acid Sequence MQVAATQRCP PQCPGRCPAT PPTCAPGVRA VLDGCSCCLV CARQRGESCS DLEPCDESSG LYCDRSADPS NQTGICTAVE GDNCVFDGVI YRSGEKFQPS CKFQCTCRDG QIGCVPRCQL DVLLPEPNCP APRKVEVPGE CCEKWICGPD EEDSLGGLTL AAYRPEATLG VEVSDSSVNC IEQTTEWTAC SKSCGMGFST RVTNRNRQCE MLKQTRLCMV RPCEQEPEQP TDKKGKKCLR TKKSLKAIHL QFKNCTSLHT YKPRFCGVCS DGRCCTPHNT KTIQAEFQCS PGQIVKKPVM VIGTCTCHTN CPKNNEAFLQ ELELKTTRGK M
Synonyms
Accession Number P48745
GeneID 4856
Summary
Source
Molecular Weight Approximately 36.2 kDa, a single non-glycosylated polypeptide chain containing 331 amino acids.
Biological Activity Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 1.0 ug/ml, corresponding to a specific activity of > 1000 IU/mg.
Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2 um filtered concentrated solution in 20 mM Tris-HCl, pH 8.6, 150 mM NaCl.
Endotoxin Less than 0.1 EU/ug of rHuNOV as determined by LAL method.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
References
SDS-PAGE SDS-PAGE of Recombinant Human Nephroblastoma-overexpressed Gene Protein Homolog
Safety Data Sheet (SDS) Download Click to download
Technical Data Sheet (TDS) Download Click to download

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