Recombinant Human Neuregulin 1-alpha EGF Domain (rHuNRG1-alpha, Q02297, 3084) CAS#: 107-20-1816; ChemWhat Code: 1405194

Official Full NameRecombinant Human Neuregulin 1-alpha EGF Domain (rHuNRG1-alpha)
SquenceSquence of Recombinant Human Neuregulin 1-alpha EGF Domain
Amino Acid SequenceSHLVKCAEKE KTFCVNGGEC FMVKDLSNPS RYLCKCQPGF TGARCTENVP MKVQNQEKAE ELYQK
Synonyms
Accession NumberQ02297
GeneID3084
Summary
Source
Molecular WeightApproximately 7.4 kDa, a single non-glycosylated polypeptide chain containing 65 amino acids.
Biological ActivityFully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 40 ng/ml, corresponding to a specific activity of > 2.5 × 104 IU/mg.
AppearanceSterile Filtered White lyophilized (freeze-dried) powder.
FormulationLyophilized from a 0.2 um filtered solution in 20 mM PB, pH 6.0, 150 mM NaCl.
EndotoxinLess than 0.1 EU/ug of rHuNRG1-alpha as determined by LAL method.
ReconstitutionWe recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability and StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
References
SDS-PAGESDS-PAGE of Recombinant Human Neuregulin 1-alpha EGF Domain
Safety Data Sheet (SDS) DownloadClick to download
Technical Data Sheet (TDS) DownloadClick to download

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