| Official Full Name |
Recombinant Human Platelet-derived Growth Factor-AA (rHuPDGF-AA) |
| Squence |
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| Amino Acid Sequence |
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| Synonyms |
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| Accession Number |
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| GeneID |
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| Summary |
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| Source |
Escherichia coli. |
| Molecular Weight |
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| Biological Activity |
Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. The ED50 for this effect is 50-200 ng/mL in a fluorometric assay using the redox sensitive dye. |
| Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation |
Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, 300 mM NaCl, pH 6.0. |
| Endotoxin |
Less than 0.1 EU/ug of rHuPDGF-AA as determined by LAL method. |
| Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Stability and Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| References |
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| SDS-PAGE |
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| Safety Data Sheet (SDS) Download |
Click to download |
| Technical Data Sheet (TDS) Download |
Click to download |
