Recombinant Human Stromal-Cell Derived Factor-1 alpha/CXCL12 alpha (rHuSDF-1alpha/CXCL12alpha, P48061, 6387) CAS#: 201-121-1816; ChemWhat Code: 1404268

Official Full NameRecombinant Human Stromal-Cell Derived Factor-1 alpha/CXCL12 alpha (rHuSDF-1alpha/CXCL12alpha)
SquenceSquence of Recombinant Human Stromal-Cell Derived Factor-1 alpha/CXCL12 alpha
Amino Acid SequenceKPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNK
SynonymsSDF-1 alpha, hSDF-1 alpha, IRH, hIRH, PBSF
Accession NumberP48061
GeneID6387
SummaryCXCL12 also known as SDF-1 is belonging to the CXC chemokine family. It is encoded by the CXCL12 gene. In recently study, Human CXCL12 is expressed as six isoforms that differ only in the C-terminal tail. And all SDF-1 isoforms undergo proteolytic processing of the first two N-terminal amino acids. Contrast to the canonical sequence SDF-1β, SDF-1alpha is shorter by four amino acids at the C-terminal tail. On the cell surface, the receptor for this chemokine is CXCR4 and syndecan4. CXCL12 is strongly chemotactic for T-lymphocytes, monocytes, but not neutrophils. CXCL12 is a very important factor in carcinogenesis and the neovascularisation linked to tumor progression.
SourceEscherichia coli.
Molecular WeightApproximately 8.0 kDa, a single non-glycosylated polypeptide chain containing 68 amino acids.
Biological ActivityFully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using PHA and rHuIL-2 activated human peripheral blood T-lymphocytes is in a concentration range of 20-80 ng/ml.
AppearanceSterile filtered white lyophilized (freeze-dried) powder.
FormulationLyophilized from a 0.2 um filtered concentrated solution in 20 mM PB pH 7.0, 130 mM NaCl.
EndotoxinLess than 1 EU/ug of rHuSDF-1α/CXCL12α as determined by LAL method.
ReconstitutionWe recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability and StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
References
SDS-PAGESDS-PAGE of Recombinant Human Stromal-Cell Derived Factor-1 alpha/CXCL12 alpha
Safety Data Sheet (SDS) DownloadClick to download
Technical Data Sheet (TDS) DownloadClick to download

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