|Official Full Name
|Recombinant Human Stromal-Cell Derived Factor-1 alpha/CXCL12 alpha (rHuSDF-1alpha/CXCL12alpha)
|Amino Acid Sequence
|KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNK
|SDF-1 alpha, hSDF-1 alpha, IRH, hIRH, PBSF
|CXCL12 also known as SDF-1 is belonging to the CXC chemokine family. It is encoded by the CXCL12 gene. In recently study, Human CXCL12 is expressed as six isoforms that differ only in the C-terminal tail. And all SDF-1 isoforms undergo proteolytic processing of the first two N-terminal amino acids. Contrast to the canonical sequence SDF-1β, SDF-1alpha is shorter by four amino acids at the C-terminal tail. On the cell surface, the receptor for this chemokine is CXCR4 and syndecan4. CXCL12 is strongly chemotactic for T-lymphocytes, monocytes, but not neutrophils. CXCL12 is a very important factor in carcinogenesis and the neovascularisation linked to tumor progression.
|Approximately 8.0 kDa, a single non-glycosylated polypeptide chain containing 68 amino acids.
|Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using PHA and rHuIL-2 activated human peripheral blood T-lymphocytes is in a concentration range of 20-80 ng/ml.
|Sterile filtered white lyophilized (freeze-dried) powder.
|Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB pH 7.0, 130 mM NaCl.
|Less than 1 EU/ug of rHuSDF-1α/CXCL12α as determined by LAL method.
|We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
|Stability and Storage
|Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
|Safety Data Sheet (SDS) Download
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|Technical Data Sheet (TDS) Download
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