Recombinant Human TNF-related Weak Inducer of Apoptosis Receptor/TNFRSF12A (rHuTWEAKReceptor/TNFRSF12A, Q9NP84, 51330) CAS#: 103-1218-1816

Official Full Name Recombinant Human TNF-related Weak Inducer of Apoptosis Receptor/TNFRSF12A (rHuTWEAKReceptor/TNFRSF12A)
Squence Squence of Recombinant Human TNF-related Weak Inducer of Apoptosis Receptor/TNFRSF12A
Amino Acid Sequence EQAPGTAPCS RGSSWSADLD KCMDCASCRA RPHSDFCLGC AAAPPAPFRL LWP
Synonyms TNFRSF12A, FGF-inducible 14, CD266
Accession Number Q9NP84
GeneID 51330
Summary
Source Escherichia coli.
Molecular Weight Approximately 5.6 kDa, a single non-glycosylated polypeptide chain containing 53 amino acids.
Biological Activity Fully biologically active when compared to standard. The ED50 as determined by inhibiting TWEAK-dependent proliferation of human umbilical vein endothelial cells (HUVEC) is less than 30 ng/ml, corresponding to a specific activity of > 3.3 × 104 IU/mg, in the presence of 15 ng/ml of rHuTWEAK.
Appearance Sterile filtered white lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Endotoxin Less than 1 EU/ug of rHuTWEAK Receptor as determined by LAL method.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
References 1. Gaudineau B, Fougere M, Guaddachi F, et al. 2012. J Cell Sci, 125: 4475-86.2. Abend JR, Uldrick T, Ziegelbauer JM. 2010. J Virol, 84: 12139-51.3. Kang S, Yang C, Luo R. 2008. Biochem Biophys Res Commun, 372: 629-33.4. Wiley SRandWinkles JA. 2003. Cytokine Growth Factor Rev, 14: 241-9.
SDS-PAGE SDS-PAGE of Recombinant Human TNF-related Weak Inducer of Apoptosis Receptor/TNFRSF12A
Safety Data Sheet (SDS) Download Click to download
Technical Data Sheet (TDS) Download Click to download

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