Official Full Name |
Recombinant Human TNF-related Weak Inducer of Apoptosis Receptor/TNFRSF12A (rHuTWEAKReceptor/TNFRSF12A) |
Squence |
 |
Amino Acid Sequence |
EQAPGTAPCS RGSSWSADLD KCMDCASCRA RPHSDFCLGC AAAPPAPFRL LWP |
Synonyms |
TNFRSF12A, FGF-inducible 14, CD266 |
Accession Number |
Q9NP84 |
GeneID |
51330 |
Summary |
|
Source |
Escherichia coli. |
Molecular Weight |
Approximately 5.6 kDa, a single non-glycosylated polypeptide chain containing 53 amino acids. |
Biological Activity |
Fully biologically active when compared to standard. The ED50 as determined by inhibiting TWEAK-dependent proliferation of human umbilical vein endothelial cells (HUVEC) is less than 30 ng/ml, corresponding to a specific activity of > 3.3 × 104 IU/mg, in the presence of 15 ng/ml of rHuTWEAK. |
Appearance |
Sterile filtered white lyophilized (freeze-dried) powder. |
Formulation |
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4. |
Endotoxin |
Less than 1 EU/ug of rHuTWEAK Receptor as determined by LAL method. |
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
Stability and Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
References |
1. Gaudineau B, Fougere M, Guaddachi F, et al. 2012. J Cell Sci, 125: 4475-86.2. Abend JR, Uldrick T, Ziegelbauer JM. 2010. J Virol, 84: 12139-51.3. Kang S, Yang C, Luo R. 2008. Biochem Biophys Res Commun, 372: 629-33.4. Wiley SRandWinkles JA. 2003. Cytokine Growth Factor Rev, 14: 241-9. |
SDS-PAGE |
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Safety Data Sheet (SDS) Download |
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Technical Data Sheet (TDS) Download |
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