| Official Full Name |
Recombinant Human Trefoil Factor 2 (rHuTFF2) |
| Squence |
 |
| Amino Acid Sequence |
EKPSPCQCSR LSPHNRTNCG FPGITSDQCF DNGCCFDSSV TGVPWCFHPL PKQESDQCVM EVSDRRNCGY PGISPEECAS RKCCFSNFIF EVPWCFFPKS VEDCHY |
| Synonyms |
Spasmolysin, Spasmolytic polypeptide, SP |
| Accession Number |
Q03403 |
| GeneID |
7032 |
| Summary |
|
| Source |
Escherichia coli. |
| Molecular Weight |
Approximately 12.0 kDa, a single non-glycosylated polypeptide chain containing 106 amino acids, which includes a 40-amino acid trefoil motif containing three conserved intramolecular disulfide bonds. |
| Biological Activity |
Fully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human MCF-7 cells is less than 10 ng/ml, corresponding to a specific activity of > 1.0 × 105 IU/mg. |
| Appearance |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation |
Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 130 mM NaCl. |
| Endotoxin |
Less than 1 EU/ug of rHuTFF2 as determined by LAL method. |
| Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Stability and Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| References |
1. Seib T, Blin N, Hilgert K, et al. 1997. Genomics, 40: 200-2.2. Kirikoshi HandKatoh M. 2002. Int J Oncol, 21: 655-9.3. Xue L, Aihara E, Podolsky DK, et al. 2010. Gut, 59: 1184-91.4. Poulsen SS, Thulesen J, Christensen L, et al. 1999. Gut, 45: 516-22. |
| SDS-PAGE |
 |
| Safety Data Sheet (SDS) Download |
Click to download |
| Technical Data Sheet (TDS) Download |
Click to download |
