Recombinant Human Trefoil Factor 2 (rHuTFF2, Q03403, 7032) CAS#: 601-15-1816

Official Full Name Recombinant Human Trefoil Factor 2 (rHuTFF2)
Squence Squence of Recombinant Human Trefoil Factor 2
Amino Acid Sequence EKPSPCQCSR LSPHNRTNCG FPGITSDQCF DNGCCFDSSV TGVPWCFHPL PKQESDQCVM EVSDRRNCGY PGISPEECAS RKCCFSNFIF EVPWCFFPKS VEDCHY
Synonyms Spasmolysin, Spasmolytic polypeptide, SP
Accession Number Q03403
GeneID 7032
Summary
Source Escherichia coli.
Molecular Weight Approximately 12.0 kDa, a single non-glycosylated polypeptide chain containing 106 amino acids, which includes a 40-amino acid trefoil motif containing three conserved intramolecular disulfide bonds.
Biological Activity Fully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human MCF-7 cells is less than 10 ng/ml, corresponding to a specific activity of > 1.0 × 105 IU/mg.
Appearance Sterile filtered white lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 130 mM NaCl.
Endotoxin Less than 1 EU/ug of rHuTFF2 as determined by LAL method.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
References 1. Seib T, Blin N, Hilgert K, et al. 1997. Genomics, 40: 200-2.2. Kirikoshi HandKatoh M. 2002. Int J Oncol, 21: 655-9.3. Xue L, Aihara E, Podolsky DK, et al. 2010. Gut, 59: 1184-91.4. Poulsen SS, Thulesen J, Christensen L, et al. 1999. Gut, 45: 516-22.
SDS-PAGE SDS-PAGE of Recombinant Human Trefoil Factor 2
Safety Data Sheet (SDS) Download Click to download
Technical Data Sheet (TDS) Download Click to download

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