Official Full Name |
Recombinant Human Tumor Necrosis Factor-beta/TNFSF1 (rHuTNF-beta/TNFSF1) |
Squence |
 |
Amino Acid Sequence |
|
Synonyms |
|
Accession Number |
|
GeneID |
|
Summary |
|
Source |
Escherichia coli. |
Molecular Weight |
|
Biological Activity |
Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 5 pg/ml, corresponding to a specific activity of > 2.0 × 108 IU/mg in the presence of actinomycin D. |
Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
Formulation |
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH7.4, with 0.02 % Tween-20. |
Endotoxin |
Less than 0.1 EU/ug of rHuTNF-beta/TNFSF1 as determined by LAL method. |
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
Stability and Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
References |
|
SDS-PAGE |
 |
Safety Data Sheet (SDS) Download |
Click to download |
Technical Data Sheet (TDS) Download |
Click to download |