UbcH10, Ubiquitin Carrier Protein C, Ubiquitin-protein Ligase C
Accession Number
GeneID
Summary
Source
Escherichia coli.
Molecular Weight
Approximately 21.1 kDa, a single non-glycosylated polypeptide chain containing 179 amino acids (a.a.) of human UBE2C/UBCH10 and 12 a.a. vector sequence including 6 × His tag at N-terminus.
Biological Activity
No data available.
Appearance
Sterile Colorless liquid.
Formulation
A 0.2 um filtered concentrated solution in 50 mM HEPES, pH 8.0, with 125 mM NaCl, 10 % Glycerol, 5 % Trehalose, 1 mM DTT.
Endotoxin
Less than 1 EU/ug of rHuUBE2C, His as determined by LAL method.
Reconstitution
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 6 months from date of receipt, -20 to -70 °C as supplied.- 3 months, -20 to -70 °C under sterile conditions after opening.
References
1. Takahashi Y, Ishii Y, Nishida Y, et al. 2006. Cancer Genet Cytogenet, 168: 30-5.2. Bavi P, Uddin S, Ahmed M, et al. 2011. Am J Pathol, 178: 2109-20.3. Chen Z, Zhang C, Wu D, et al. 2011. EMBO J, 30: 2405-19.4. Hao Z, Zhang H, Cowell J. 2012. Tumour Biol, 33: 723-30.