| Official Full Name |
Recombinant Human Ubiquitin-conjugating Enzyme E2 L3, His (rHuUBE2L3,His) |
| Squence |
 |
| Amino Acid Sequence |
MHHHHHHAMA ASRRLMKELE EIRKCGMKNF RNIQVDEANL LTWQGLIVPD NPPYDKGAFR IEINFPAEYP FKPPKITFKT KIYHPNIDEK GQVCLPVISA ENWKPATKTD QVIQSLIALV NDPQPEHPLR ADLAEEYSKD RKKFCKNAEE FTKKYGEKRP VD |
| Synonyms |
L-UBC, UbcH7, Ubiquitin Carrier Protein L3, Ubiquitin-protein Ligase L3 |
| Accession Number |
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| GeneID |
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| Summary |
|
| Source |
Escherichia coli. |
| Molecular Weight |
Approximately 18.9 kDa, a single non-glycosylated polypeptide chain containing 154 amino acids (a.a.) of human UBE2L3/UBCH7 and 8 a.a. vector sequence including 6 × His tag at N-terminus. |
| Biological Activity |
No data available. |
| Appearance |
Sterile Colorless liquid. |
| Formulation |
A 0.2 um filtered concentrated solution in 50 mM HEPES, pH 7.0, with 125 mM NaCl, 10 % Glycerol, 5 % Trehalose, 1 mM DTT. |
| Endotoxin |
Less than 1 EU/ug of rHuUBE2L3, His as determined by LAL method. |
| Reconstitution |
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| Stability and Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 6 months from date of receipt, -20 to -70 °C as supplied.- 3 months, -20 to -70 °C under sterile conditions after opening. |
| References |
1. Serniwka SAandShaw GS. 2008. Biomol NMR Assign, 2: 21-3.2. Wenzel DM, Lissounov A, Brzovic PS, et al. 2011. Nature, 474: 105-8.3. Whitcomb EAandTaylor A. 2009. Cell Div, 4: 17.4. Moynihan TP, Ardley HC, Nuber U, et al. 1999. J Biol Chem, 274: 30963-8. |
| SDS-PAGE |
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| Safety Data Sheet (SDS) Download |
Click to download |
| Technical Data Sheet (TDS) Download |
Click to download |
