Recombinant Human Vascular Endothelial Growth Factor 165 (rHuVEGF165, P15692, 7422) CAS#: 105-05-1816; ChemWhat Code: 1404466

Official Full Name Recombinant Human Vascular Endothelial Growth Factor 165 (rHuVEGF165)
Squence Squence of Recombinant Human Vascular Endothelial Growth Factor 165
Amino Acid Sequence MAPMAEGGGQ NHHEVVKFMD VYQRSYCHPI ETLVDIFQEY PDEIEYIFKP SCVPLMRCGG CCNDEGLECV PTEESNITMQ IMRIKPHQGQ HIGEMSFLQH NKCECRPKKD RARQENPCGP CSERRKHLFV QDPQTCKCSC KNTDSRCKAR QLELNERTCR CDKPRR
Synonyms Vascular Endothelial Growth Factor Isoform 165
Accession Number P15692
GeneID 7422
Summary
Source Escherichia coli.
Molecular Weight Approximately 38.6 kDa, a disulfide-linked homodimeric protein consisting of two 166 amino acid polypeptide chains with Met at N-terminus.
Biological Activity Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human umbilical vein endothelial cells(HUVEC) is between 1.0-8.0 ng/ml.
Appearance Sterile filtered white lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Endotoxin Less than 1 EU/ug of rHuVEGF165 as determined by LAL method.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
References 1. Leung DW, Cachianes G, Kuang WJ, et al. 1989. Science. 246:1306-9.2. Byrne AM, Bouchier-Hayes DJ, Harmey JH. 2005. J Cell Mol Med. 9:777-94.3. Robinson CJ, Stringer SE. 2001. J Cell Sci. 114:853-65.
SDS-PAGE SDS-PAGE of Recombinant Human Vascular Endothelial Growth Factor 165
Safety Data Sheet (SDS) Download Click to download
Technical Data Sheet (TDS) Download Click to download

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