Official Full Name |
Recombinant Murine Beta-defensin 1 (rMuBD-1) |
Squence |
 |
Amino Acid Sequence |
VGILTSLGRR TDQYKCLQHG GFCLRSSCPS NTKLQGTCKP DKPNCCKS |
Synonyms |
Defensin beta1 |
Accession Number |
P56386 |
GeneID |
13214 |
Summary |
|
Source |
Escherichia coli. |
Molecular Weight |
Approximately 5.2 kDa, a single non-glycosylated polypeptide chain containing 48 amino acids. |
Biological Activity |
Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using CD34+ dendritic cells is in a concentration range of 100.0-1000.0 ng/ml. |
Appearance |
Sterile filtered white lyophilized (freeze-dried) powder. |
Formulation |
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4. |
Endotoxin |
Less than 1 EU/ug of rMuBD-1 as determined by LAL method. |
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
Stability and Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
References |
1. Wang YS, Wang GQ, Wen YJ, et al. 2007. Clin Cancer Res, 13: 6779-87.2. Morrison GM, Davidson DJ, Kilanowski FM, et al. 1998. Mamm Genome, 9: 453-7.3. Huttner KM, Kozak CA, Bevins CL. 1997. FEBS Lett, 413: 45-9.4. Yudin AI, Tollner TL, Treece CA, et al. 2008. Reproduction, 136: 753-65. |
SDS-PAGE |
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Safety Data Sheet (SDS) Download |
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Technical Data Sheet (TDS) Download |
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