| Official Full Name |
Recombinant Murine Breast and Kindey-expressed Chemokine/CXCL14 (rMuBRAK/CXCL14) |
| Squence |
 |
| Amino Acid Sequence |
SKCKCSRKGP KIRYSDVKKL EMKPKYPHCE EKMVIVTTKS MSRYRGQEHC LHPKLQSTKR FIKWYNAWNE KRRVYEE |
| Synonyms |
B-cell and Monocyte-activating Chemokine, Chemokine BRAK, Kidney-expressed Chemokine CXC, MIP-2G, Small-inducible Cytokine B14 |
| Accession Number |
Q9WUQ5 |
| GeneID |
57266 |
| Summary |
|
| Source |
Escherichia coli. |
| Molecular Weight |
Approximately 9.4 kDa, a single non-glycosylated polypeptide chain containing 77amino acids. |
| Biological Activity |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 1.0-10 ng/ml. |
| Appearance |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation |
Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, 400 mM NaCl, pH 7.4, 5 % trehalose. |
| Endotoxin |
Less than 1 EU/ug of rMuBRAK/CXCL14 as determined by LAL method. |
| Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Stability and Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| References |
1. Hromas R, Broxmeyer HE, Kim C, et al. 1999. Biochem Biophys Res Commun. 255:703-6.2. Starnes T, Rasila KK, Robertson MJ, et al. 2006. Exp Hematol. 34:1101-5.3. Shurin GV, Ferris RL, Tourkova IL, et al. 2005. J Immunol. 174:5490-8.4. Kurth I, Willimann K, Schaerli P, et al. 2001. J Exp Med. 194:855-61.5. Hara T, Tanegashima K. 2012. J Biochem. 151:469-76.6. Shellenberger TD, Wang M, Gujrati M, et al. 2004. Cancer Res. 64:8262-70. |
| SDS-PAGE |
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| Safety Data Sheet (SDS) Download |
Click to download |
| Technical Data Sheet (TDS) Download |
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