Recombinant Murine Desert Hedgehog Homolog Cys23IleIle (rMuDHHC23II, Q61488, 13363) CAS#: 621-401-1816; ChemWhat Code: 1404932

Official Full Name Recombinant Murine Desert Hedgehog Homolog Cys23IleIle (rMuDHHC23II)
Squence Squence of Recombinant Murine Desert Hedgehog Homolog Cys23IleIle
Amino Acid Sequence IIGPGRGPVG RRRYVRKQLV PLLYKQFVPS MPERTLGASG PAEGRVTRGS ERFRDLVPNY NPDIIFKDEE NSGADRLMTE RCKERVNALA IAVMNMWPGV RLRVTEGWDE DGHHAQDSLH YEGRALDITT SDRDRNKYGL LARLAVEAGF DWVYYESRNH IHVSVKADNS LAVRAGG
Synonyms
Accession Number Q61488
GeneID 13363
Summary
Source
Molecular Weight Approximately 20.0 kDa, a single non-glycosylated polypeptide chain containing 177 amino acids.
Biological Activity Fully biologically active when compared to standard. The ED50 as determined by its ability to induce alkaline phosphatase production by murine MC3T3-E1 cells is 5-20 ug/ml.
Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4, 1 mM DTT, 0.05 % Tween-80.
Endotoxin Less than 0.1 EU/ug of rMuDHH C23II as determined by LAL method.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
References
SDS-PAGE SDS-PAGE of Recombinant Murine Desert Hedgehog Homolog Cys23IleIle
Safety Data Sheet (SDS) Download Click to download
Technical Data Sheet (TDS) Download Click to download

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