Recombinant Murine Growth Differentiation Factor 7/Bone Morphogenetic Protein-12 (rMuGDF-7/BMP-12, P43029, 238057) CAS#: 128-12-1816; ChemWhat Code: 1404898

Official Full Name Recombinant Murine Growth Differentiation Factor 7/Bone Morphogenetic Protein-12 (rMuGDF-7/BMP-12)
Squence Squence of Recombinant Murine Growth Differentiation Factor 7/Bone Morphogenetic Protein-12
Amino Acid Sequence TALAGTRGAQ GSGGGGGGGG GGGGGGGGGG GGAGRGHGRR GRSRCSRKSL HVDFKELGWD DWIIAPLDYE AYHCEGVCDF PLRSHLEPTN HAIIQTLLNS MAPDAAPASC CVPARLSPIS ILYIDAANNV VYKQYEDMVV EACGCR
Synonyms
Accession Number P43029
GeneID 238057
Summary
Source
Molecular Weight Approximately 29.8 kDa, a homodimeric protein consisting of two 146 amino acid non-glycosylated polypeptide chains.
Biological Activity Fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine ATDC5 cells is less than 0.5 ug/ml, corresponding to a specific activity of > 2000 IU/mg.
Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2 um filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA.
Endotoxin Less than 0.1 EU/ug of rMuGDF-7/BMP-12 as determined by LAL method.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
References
SDS-PAGE SDS-PAGE of Recombinant Murine Growth Differentiation Factor 7/Bone Morphogenetic Protein-12
Safety Data Sheet (SDS) Download Click to download
Technical Data Sheet (TDS) Download Click to download

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