Recombinant Murine Interleukin-1 Receptor Antagonist Protein (rMuIL-1RA, Q542W1, 16181) CAS#: 121-013-1816; ChemWhat Code: 1404686

Official Full Name Recombinant Murine Interleukin-1 Receptor Antagonist Protein (rMuIL-1RA)
Squence Squence of Recombinant Murine Interleukin-1 Receptor Antagonist Protein
Amino Acid Sequence RPSGKRPCKM QAFRIWDTNQ KTFYLRNNQL IAGYLQGPNI KLEEKIDMVP IDLHSVFLGI HGGKLCLSCA KSGDDIKLQL EEVNITDLSK NKEEDKRFTF IRSEKGPTTS FESAACPGWF LCTTLEADRP VSLTNTPEEP LIVTKFYFQE DQ
Synonyms IL-1RN, IRAP
Accession Number Q542W1
GeneID 16181
Summary IL-1RA was initially called the IL-1 inhibitor which is encoded by the IL1RN gene and it is a member of the interleukin 1 cytokine family. IL-1RA is secreted by various types of cells including immune cells, epithelial cells, and adipocytes. IL-RA has functions of inhibiting the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. IL-1RA is also used in the treatment of rheumatoid arthritis, an autoimmune disease in which IL-1 plays a key role. The murine IL-1RA is a single non-glycosylated polypeptide chain containing 152 amino acids and it has been shown to block the inflammatory responses induced by IL-1 both in vitro and in vivo. The protein shows 26 % amino acid homology to IL-1β and 19% homology to IL-1alpha. It also shares 90 % a.a. sequence identity with rat IL-1RA.
Source Escherichia coli.
Molecular Weight Approximately 17.3 kDa, a single non-glycosylated polypeptide chain containing 152 amino acids.
Biological Activity Fully biologically active when compared to standard. The ED50 as determined by inhibiting IL-1α-dependent proliferation of murine D10S cells is less than 50 ng/ml, corresponding to a specific activity of > 2.0 × 104 IU/mg in the presence of 50 pg/ml rHuIL-1α.
Appearance Sterile filtered white lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Endotoxin Less than 1 EU/ug of rMuIL-1RA as determined by LAL method.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
References
SDS-PAGE SDS-PAGE of Recombinant Murine Interleukin-1 Receptor Antagonist Protein
Safety Data Sheet (SDS) Download Click to download
Technical Data Sheet (TDS) Download Click to download

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