Recombinant Murine KC/CXCL1 (rMuKC/CXCL1, P12850, 14825) CAS#: 221-01-1816; ChemWhat Code: 1404222

Official Full Name Recombinant Murine KC/CXCL1 (rMuKC/CXCL1)
Squence Squence of Recombinant Murine KC/CXCL1
Amino Acid Sequence APIANELRCQ CLQTMAGIHL KNIQSLKVLP SGPHCTQTEV IATLKNGREA CLDPEAPLVQ KIVQKMLKGV PK
Synonyms C-X-C motif chemokine 1, GRO-alpha, Platelet-derived growth factor-inducible protein, KC, Secretory protein N51
Accession Number P12850
GeneID 14825
Summary Murine CXCL1, also known as KC, is belonging to the CXC chemokine family. It is encoded by the GRO gene now designated CXCL1. The gene for CXCL1 was initially discovered in mouse fibroblasts by plateletderived growth factor. KC is member of the intercrine alpha (chemokine C-X-C) subfamily of chemokines. It is secreted by human melanoma cells, and also expressed by macrophages, neutrophils and epithelial cells. The functional receptor for CXCL1 has been identified as CXCR2. CXCL1 has chemotactic activity for neutrophils, and plays a role in inflammation and wound healing. Amino acid sequence of murine CXCL1 is approximately 60 % identical to the human CXCL1. KC was found to be involved in monocyte arrest on atherosclerotic endothelium and may also play a pathophysiological role in Alzheimer’s disease.
Source Escherichia coli.
Molecular Weight Approximately 7.8 kDa, a single non-glycosylated polypeptide chain containing 72 amino acid residues.
Biological Activity Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood neutrophils is in a concentration range of 10-100 ng/ml.
Appearance Sterile filtered white lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Endotoxin Less than 1 EU/ug of rMuKC/CXCL1 as determined by LAL method.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
References
SDS-PAGE SDS-PAGE of Recombinant Murine KC/CXCL1
Safety Data Sheet (SDS) Download Click to download
Technical Data Sheet (TDS) Download Click to download

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