| Official Full Name |
Recombinant Murine Macrophage Inflammatory Protein-1 beta/CCL4 (rMuMIP-1beta/CCL4) |
| Squence |
 |
| Amino Acid Sequence |
APMGSDPPTS CCFSYTSRQL HRSFVMDYYE TSSLCSKPAV VFLTKRGRQI CANPSEPWVT EYMSDLELN |
| Synonyms |
ACT-2, MIP-1-beta, Protein H400, SIS-gamma, Small-inducible cytokine A4 |
| Accession Number |
P14097 |
| GeneID |
20303 |
| Summary |
Chemokine (C-C motif) ligand 4, also known as Macrophage inflammatory protein-1β (MIP-1β) is a CC chemokine with specificity for CCR5 receptors and it is a major HIV-suppressive factor produced by CD8+ T cells. In addition, it is a monokine with inflammatory and chemokinetic properties. Recombinant CCL4 induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). Furthermore, recombinant murine CCL4 contains 69 amino acids and it shares 77 % and 86 % a.a. sequence identity with human and rat CCL4. Both human and murine MIP-1alpha and MIP-1β are active on human and murine hematopoietic cells. |
| Source |
Escherichia coli. |
| Molecular Weight |
Approximately 7.8 kDa, a single non-glycosylated polypeptide chain containing 69 amino acids. |
| Biological Activity |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 20-100 ng/ml. |
| Appearance |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation |
Lyophilized from a 0.2 um filtered concentrated solution in 2 × PBS, pH 7.4. |
| Endotoxin |
Less than 1 EU/ug of rMuMIP-1β/CCL4 as determined by LAL method. |
| Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Stability and Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| References |
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| SDS-PAGE |
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| Safety Data Sheet (SDS) Download |
Click to download |
| Technical Data Sheet (TDS) Download |
Click to download |
