| Official Full Name |
Recombinant Murine Monocyte Chemotactic Protein-5/CCL12 (rMuMCP-5/CCL12) |
| Squence |
 |
| Amino Acid Sequence |
GPDAVSTPVT CCYNVVKQKI HVRKLKSYRR ITSSQCPREA VIFRTILDKE ICADPKEKWV KNSINHLDKT SQTFILEPSC LG |
| Synonyms |
MCP-1-related Chemokine, Small-inducible Cytokine A12 |
| Accession Number |
Q62401 |
| GeneID |
20293 |
| Summary |
|
| Source |
Escherichia coli. |
| Molecular Weight |
Approximately 9.3 kDa, a single non-glycosylated polypeptide chain containing 82 amino acids. |
| Biological Activity |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 10-50 ng/ml. |
| Appearance |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation |
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4. |
| Endotoxin |
Less than 1 EU/ug of rMuMCP-5/CCL12 as determined by LAL method. |
| Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Stability and Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| References |
1. Sarafi MN, Garcia-Zepeda EA, MacLean JA, et al. 1997. J Exp Med. 185:99-109.2. Jia GQ, Gonzalo JA, Lloyd C, et al. 1996. J Exp Med. 184:1939-51. |
| SDS-PAGE |
 |
| Safety Data Sheet (SDS) Download |
Click to download |
| Technical Data Sheet (TDS) Download |
Click to download |
