| Official Full Name |
Recombinant Murine Noggin (rMuNoggin) |
| Squence |
 |
| Amino Acid Sequence |
MQHYLHIRPA PSDNLPLVDL IEHPDPIFDP KEKDLNETLL RSLLGGHYDP GFMATSPPED RPGGGGGPAG GAEDLAELDQ LLRQRPSGAM PSEIKGLEFS EGLAQGKKQR LSKKLRRKLQ MWLWSQTFCP VLYAWNDLGS RFWPRYVKVG SCFSKRSCSV PEGMVCKPSK SVHLTVLRWR CQRRGGQRCG WIPIQYPIIS ECKCSC |
| Synonyms |
|
| Accession Number |
P97466 |
| GeneID |
18121 |
| Summary |
|
| Source |
Escherichia coli. |
| Molecular Weight |
Approximately 46.4 kDa, a disulfide-linked homodimer consisting of two 206 amino acid polypeptide chains. |
| Biological Activity |
Fully biologically active when compared to standard. The ED50 as determined by inhibiting BMP-4-induced alkaline phosphatase production of murine ATDC5 cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 × 105 IU/mg in the presence of 5 ng/ml BMP-4. |
| Appearance |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation |
Lyophilized from a 0.2 um filtered concentrated solution in 30 % acetonitrile, 0.1 % TFA. |
| Endotoxin |
Less than 1 EU/ug of rMuNoggin as determined by LAL method. |
| Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 10 mM HAc to a concentration less than 0.25 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Stability and Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| References |
1. Davis SWandCamper SA. 2007. Dev Biol, 305: 145-60.2. Zhu W, Kim J, Cheng C, et al. 2006. Bone, 39: 61-71.3. Oxley CD, Rashid R, Goudie DR, et al. 2008. Horm Res, 69: 221-6.4. Cooper GM, Usas A, Olshanski A, et al. 2009. Plast Reconstr Surg, 123: 94S-103S.5. Bayramov AV, Eroshkin FM, Martynova NY, et al. 2011. Development, 138: 5345-56. |
| SDS-PAGE |
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| Safety Data Sheet (SDS) Download |
Click to download |
| Technical Data Sheet (TDS) Download |
Click to download |
