Recombinant Murine TNF-related apoptosis-inducing Ligand/TNFSF10 (rMuTRAIL/TNFSF10, P50592, 22035) CAS#: 123-15-1816

Official Full Name Recombinant Murine TNF-related apoptosis-inducing Ligand/TNFSF10 (rMuTRAIL/TNFSF10)
Squence Squence of Recombinant Murine TNF-related apoptosis-inducing Ligand/TNFSF10
Amino Acid Sequence MPRGGRPQKV AAHITGITRR SNSALIPISK DGKTLGQKIE SWESSRKGHS FLNHVLFRNG ELVIEQEGLY YIYSQTYFRF QEAEDASKMV SKDKVRTKQL VQYIYKYTSY PDPIVLMKSA RNSCWSRDAE YGLYSIYQGG LFELKKNDRI FVSVTNEHLM DLDQEASFFG AFLIN
Synonyms
Accession Number P50592
GeneID 22035
Summary
Source
Molecular Weight Approximately 20.2 kDa, a single non-glycosylated polypeptide chain containing 175 amino acids.
Biological Activity Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 106 IU/mg in the presence of actinomycin D.
Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4, with 3 mM DTT.
Endotoxin Less than 0.1 EU/ug of rMuTRAIL/TNFSF10 as determined by LAL method.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
References
SDS-PAGE SDS-PAGE of Recombinant Murine TNF-related apoptosis-inducing Ligand/TNFSF10
Safety Data Sheet (SDS) Download Click to download
Technical Data Sheet (TDS) Download Click to download

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