Recombinant Porcine Interleukin-1 Receptor Antagonist Protein (rPoIL-1RA, Q29056, 397499) CAS#: 171-013-1816

Official Full Name Recombinant Porcine Interleukin-1 Receptor Antagonist Protein (rPoIL-1RA)
Squence Squence of Recombinant Porcine Interleukin-1 Receptor Antagonist Protein
Amino Acid Sequence HPLGKRPCRM QAFRIWDVNQ KTFYLRNNQL VAGYLQGPNT KLEEKIDVVP VEPHFVFLGI HGGKLCLSCV KSGDEMKLQL DAVNITDLRK NSEQDKRFTF IRSDSGPTTS FESAACPGWF LCTALEADQP VGLTNTPKAA VKVTKFYFQQ DQ
Synonyms
Accession Number Q29056
GeneID 397499
Summary
Source
Molecular Weight Approximately 17.1 kDa, a single non-glycosylated polypeptide chain containing 152 amino acids.
Biological Activity Fully biologically active when compared to standard. The ED50 as determined by inhibiting IL-1alpha-dependent proliferation of murine D10S cells is less than 50 ng/ml, corresponding to a specific activity of > 2.0 × 104 IU/mg in the presence of 75 pg/ml rPoIL-1alpha.
Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2 um filtered concentrated solution in 1 × PBS, pH 7.4, 1 mM DTT.
Endotoxin Less than 0.1 EU/ug of rPoIL-1RA as determined by LAL method.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
References
SDS-PAGE SDS-PAGE of Recombinant Porcine Interleukin-1 Receptor Antagonist Protein
Safety Data Sheet (SDS) Download Click to download
Technical Data Sheet (TDS) Download Click to download

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