| Official Full Name |
Recombinant Rat Beta-defensin 1 (rRtBD-1) |
| Squence |
 |
| Amino Acid Sequence |
DQYRCLQNGG FCLRSSCPSH TKLQGTCKPD KPNCCRS |
| Synonyms |
Defensin beta1 |
| Accession Number |
O89117 |
| GeneID |
83687 |
| Summary |
|
| Source |
Escherichia coli. |
| Molecular Weight |
Approximately 4.1 kDa, a single non-glycosylated polypeptide chain containing 37 amino acids. |
| Biological Activity |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using CD34+ dendritic cells is in a concentration range of 100.0-1000.0 ng/ml. |
| Appearance |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation |
Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, 500 mM NaCl, pH 7.0. |
| Endotoxin |
Less than 1 EU/ug of rRtBD-1 as determined by LAL method. |
| Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Stability and Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| References |
1. Wang YS, Wang GQ, Wen YJ, et al. 2007. Clin Cancer Res, 13: 6779-87.2. Morrison GM, Davidson DJ, Kilanowski FM, et al. 1998. Mamm Genome, 9: 453-7.3. Kurosawa S, Ohta M, Hayakawa M, et al. 2002. Hybrid Hybridomics, 21: 359-63.4. Palladino MA, Mallonga TA, Mishra MS. 2003. Biol Reprod, 68: 509-15.5. Kurland AR, Schreiner H, Diamond G. 2006. J Periodontal Res, 41: 567-72. |
| SDS-PAGE |
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| Safety Data Sheet (SDS) Download |
Click to download |
| Technical Data Sheet (TDS) Download |
Click to download |
