Recombinant Rat Beta-defensin 3 (rRtBD-3, Q32ZI4, 641623) CAS#: 149-03-1816; ChemWhat Code: 1404628

Official Full NameRecombinant Rat Beta-defensin 3 (rRtBD-3)
SquenceSquence of Recombinant Rat Beta-defensin 3
Amino Acid SequenceKKVYNAVSCM TNGGICWLKC SGTFREIGSC GTRQLKCCKK K
SynonymsDefensin beta3
Accession NumberQ32ZI4
GeneID641623
SummaryDefensins (alpha and beta) are cationic peptides with antimicrobial activity against Gram-negative and Gram-positive bacteria, fungi, and enveloped viruses. They are 2-6 kDa proteins and take important roles in innate immune system. On the basis of their size and pattern of disulfide bonding, mammalian defensins are classified into alpha, beta and theta categories. β-Defensins are expressed on some leukocytes and at epithelial surfaces. They contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Because β-defensins are cationic peptides, they can therefore interact with the membrane of invading microbes, which are negative due to lipopolysaccharides (LPS) and lipoteichoic acid (LTA) found in the cell membrane. Especially, they have higher affinity to the binding site compared to Ca2+ and Mg2+ ions. Furthermore, they can affect the stability of the membrane. Additionally, they are not only have the ability to strengthen the innate immune system but can also enhance the adaptive immune system by chemotaxis of monocytes, T-lymphocytes, dendritic cells and mast cells to the infection site.
SourceEscherichia coli.
Molecular WeightApproximately 4.5 kDa, a single non-glycosylated polypeptide chain containing 41 amino acids.
Biological ActivityFully biologically active when compared to standard. Measured by its antimicrobial activity against E. coli. The ED50 for this effect is typically 4-20 ug/ml.
AppearanceSterile filtered white lyophilized (freeze-dried) powder.
FormulationLyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
EndotoxinLess than 1 EU/ug of rRtBD-3 as determined by LAL method.
ReconstitutionWe recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability and StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
References
SDS-PAGESDS-PAGE of Recombinant Rat Beta-defensin 3
Safety Data Sheet (SDS) DownloadClick to download
Technical Data Sheet (TDS) DownloadClick to download

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