| Official Full Name |
Recombinant Rat Cytokine-induced Neutrophil Chemoattractant 1/CXCL1 (rRtCINC-1/CXCL1) |
| Squence |
 |
| Amino Acid Sequence |
APVANELRCQ CLQTVAGIHF KNIQSLKVMP PGPHCTQTEV IATLKNGREA CLDPEAPMVQ KIVQKMLKGV PK |
| Synonyms |
GRO-alpha, Platelet-derived Growth Factor-inducible Protein KC |
| Accession Number |
P14095 |
| GeneID |
81503 |
| Summary |
|
| Source |
Escherichia coli. |
| Molecular Weight |
Approximately 7.8 kDa, a single, non-glycosylated polypeptide chain containing 72 amino acids. |
| Biological Activity |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using rat neutrophils is in a concentration range of 10-100 ng/ml. |
| Appearance |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation |
Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl. |
| Endotoxin |
Less than 1 EU/ug of rRtCINC-1/CXCL1 as determined by LAL method. |
| Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Stability and Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| References |
1. Haskill S, Peace A, Morris J, et al. 1990. Proc Natl Acad Sci U S A. 87:7732-6.2. Richmond A, Thomas HG. 1988. J Cell Biochem. 36:185-98.3. Iida N, Grotendorst GR. 1990. Mol Cell Biol. 10:5596-9.4. Tsai HH, Frost E, To V, et al. 2002. Cell. 110:373-83.5. Moser B, Clark-Lewis I, Zwahlen R, et al. 1990. J Exp Med. 171:1797-802.6. Devalaraja RM, Nanney LB, Du J, et al. 2000. J Invest Dermatol. 115:234-44. |
| SDS-PAGE |
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| Safety Data Sheet (SDS) Download |
Click to download |
| Technical Data Sheet (TDS) Download |
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