| Official Full Name |
Recombinant Rat Cytokine-induced Neutrophil Chemoattractant-2 beta/CXCL3 (rRtCINC-2beta/CXCL3) |
| Squence |
 |
| Amino Acid Sequence |
RELRCQCLKT LPRVDFENIQ SLTVTPPGPH CTQTEVIATL KDGQEVCLNP QAPRLQKIIQ KLLKSPSL |
| Synonyms |
CINC-2beta, MIP2-beta |
| Accession Number |
Q10746 |
| GeneID |
171551 |
| Summary |
|
| Source |
Escherichia coli. |
| Molecular Weight |
Approximately 7.6 kDa, a single non-glycosylated polypeptide chain containing 68 amino acids. |
| Biological Activity |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human CXCR2 transfected murine BaF3 cells is in a concentration range of 5-50 ng/ml. |
| Appearance |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation |
Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 50 mM NaCl. |
| Endotoxin |
Less than 1 EU/ug of rRtCINC-2β/CXCL3 as determined by LAL method. |
| Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Stability and Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| References |
1. Nolan KF, Strong V, Soler D, et al. 2004. J Immunol. 172:2201-9.2. Modi WS, Yoshimura T. 1999. Mol Biol Evol. 16:180-93.3. Tsai HH, Frost E, To V, et al. 2002. Cell. 110:373-83.4. Smith DF, Galkina E, Ley K, et al. 2005. Am J Physiol Heart Circ Physiol. 289:H1976-84.5. Nakagawa H, Komorita N, Shibata F, et al. 1994. Biochem J. 301 ( Pt 2):545-50. |
| SDS-PAGE |
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| Safety Data Sheet (SDS) Download |
Click to download |
| Technical Data Sheet (TDS) Download |
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