Recombinant Rat Cytokine-induced Neutrophil Chemoattractant-2 beta/CXCL3 (rRtCINC-2beta/CXCL3, Q10746, 171551) CAS#: 241-032-1816

Official Full Name Recombinant Rat Cytokine-induced Neutrophil Chemoattractant-2 beta/CXCL3 (rRtCINC-2beta/CXCL3)
Squence Squence of Recombinant Rat Cytokine-induced Neutrophil Chemoattractant-2 beta/CXCL3
Amino Acid Sequence RELRCQCLKT LPRVDFENIQ SLTVTPPGPH CTQTEVIATL KDGQEVCLNP QAPRLQKIIQ KLLKSPSL
Synonyms CINC-2beta, MIP2-beta
Accession Number Q10746
GeneID 171551
Summary
Source Escherichia coli.
Molecular Weight Approximately 7.6 kDa, a single non-glycosylated polypeptide chain containing 68 amino acids.
Biological Activity Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human CXCR2 transfected murine BaF3 cells is in a concentration range of 5-50 ng/ml.
Appearance Sterile filtered white lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 50 mM NaCl.
Endotoxin Less than 1 EU/ug of rRtCINC-2β/CXCL3 as determined by LAL method.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
References 1. Nolan KF, Strong V, Soler D, et al. 2004. J Immunol. 172:2201-9.2. Modi WS, Yoshimura T. 1999. Mol Biol Evol. 16:180-93.3. Tsai HH, Frost E, To V, et al. 2002. Cell. 110:373-83.4. Smith DF, Galkina E, Ley K, et al. 2005. Am J Physiol Heart Circ Physiol. 289:H1976-84.5. Nakagawa H, Komorita N, Shibata F, et al. 1994. Biochem J. 301 ( Pt 2):545-50.
SDS-PAGE SDS-PAGE of Recombinant Rat Cytokine-induced Neutrophil Chemoattractant-2 beta/CXCL3
Safety Data Sheet (SDS) Download Click to download
Technical Data Sheet (TDS) Download Click to download

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