Official Full Name |
Recombinant Rat Granulocyte Colony-Stimulating Factor (rRtG-CSF) |
Squence |
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Amino Acid Sequence |
KKIPLLTVSS LPPSLPLPRS FLLKSLEQVR KIQARNTELL EQLCATYKLC HPEELVLFGH SLGIPKASLS SCSSQALQQT KCLSQLHSGL FLYQGLLQAL AGISSELAPT LDMLHLDVDN FATTIWQQME SLGVAPTVQP TQSTMPIFTS AFQRRAGGVL VTSYLQSFLE TAHHALHHLP RPAQKHFPES LFISI |
Synonyms |
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Accession Number |
P97712 |
GeneID |
25610 |
Summary |
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Source |
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Molecular Weight |
Approximately 21.5 kDa, a single non-glycosylated polypeptide chain containing 195 amino acids. |
Biological Activity |
Fully biologically active when compared to standard. The ED50 determined by a cell proliferation assay using murine NFS-60 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 × 107 IU/mg. |
Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
Formulation |
Lyophilized from a 0.2 um filtered concentrated solution in 5 mM Sodium Citrate, pH 4.0. |
Endotoxin |
Less than 0.1 EU/ug of rRtG-CSF as determined by LAL method. |
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
Stability and Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
References |
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SDS-PAGE |
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Safety Data Sheet (SDS) Download |
Click to download |
Technical Data Sheet (TDS) Download |
Click to download |