Recombinant Rat Granulocyte Colony-Stimulating Factor (rRtG-CSF, P97712, 25610) CAS#: 142-02-1816; ChemWhat Code: 1405108

Official Full Name Recombinant Rat Granulocyte Colony-Stimulating Factor (rRtG-CSF)
Squence Squence of Recombinant Rat Granulocyte Colony-Stimulating Factor
Amino Acid Sequence KKIPLLTVSS LPPSLPLPRS FLLKSLEQVR KIQARNTELL EQLCATYKLC HPEELVLFGH SLGIPKASLS SCSSQALQQT KCLSQLHSGL FLYQGLLQAL AGISSELAPT LDMLHLDVDN FATTIWQQME SLGVAPTVQP TQSTMPIFTS AFQRRAGGVL VTSYLQSFLE TAHHALHHLP RPAQKHFPES LFISI
Synonyms
Accession Number P97712
GeneID 25610
Summary
Source
Molecular Weight Approximately 21.5 kDa, a single non-glycosylated polypeptide chain containing 195 amino acids.
Biological Activity Fully biologically active when compared to standard. The ED50 determined by a cell proliferation assay using murine NFS-60 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 × 107 IU/mg.
Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2 um filtered concentrated solution in 5 mM Sodium Citrate, pH 4.0.
Endotoxin Less than 0.1 EU/ug of rRtG-CSF as determined by LAL method.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
References
SDS-PAGE SDS-PAGE of Recombinant Rat Granulocyte Colony-Stimulating Factor
Safety Data Sheet (SDS) Download Click to download
Technical Data Sheet (TDS) Download Click to download

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