| Official Full Name |
Recombinant Rat Interleukin-33 (rRtIL-33) |
| Squence |
 |
| Amino Acid Sequence |
SIQGTSLLTE SCALSTYNDQ SVSFVLENGC YVINVEDCGK NQEKDKVLLR YYESSFPAQS GDGVDGKKLM VNMSPIKDTD IWLNANDKDY SVELQKGDVS PPDQAFFVLH KKSSDFVSFE CKNLPGTYIG VKDNQLALVE ENDESCNNIM FKLSKM |
| Synonyms |
IL-33 |
| Accession Number |
Q66H70 |
| GeneID |
361749 |
| Summary |
|
| Source |
Escherichia coli. |
| Molecular Weight |
Approximately 17.4 kDa, a single non-glycosylated polypeptide chain containing 156 amino acids. |
| Biological Activity |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 106 IU/mg. |
| Appearance |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation |
Lyophilized from a 0.2 um filtered concentrated solution in 20 mM Tris, 300 mM NaCl, pH 8.5. |
| Endotoxin |
Less than 1 EU/ug of rRtIL-33 as determined by LAL method. |
| Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Stability and Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| References |
1. Lingel A, Weiss TM, Niebuhr M, et al. 2009. Structure. 17:1398-410.2. Baekkevold ES, Roussigne M, Yamanaka T, et al. 2003. Am J Pathol. 163:69-79.3. Onda H, Kasuya H, Takakura K, et al. 1999. J Cereb Blood Flow Metab. 19:1279-88.4. Black RA, Kronheim SR, Merriam JE, et al. 1989. J Biol Chem. 264:5323-6. |
| SDS-PAGE |
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| Safety Data Sheet (SDS) Download |
Click to download |
| Technical Data Sheet (TDS) Download |
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