Recombinant Rat Interleukin-33 (rRtIL-33, Q66H70, 361749) CAS#: 141-33-1816; ChemWhat Code: 1404640

Official Full Name Recombinant Rat Interleukin-33 (rRtIL-33)
Squence Squence of Recombinant Rat Interleukin-33
Amino Acid Sequence SIQGTSLLTE SCALSTYNDQ SVSFVLENGC YVINVEDCGK NQEKDKVLLR YYESSFPAQS GDGVDGKKLM VNMSPIKDTD IWLNANDKDY SVELQKGDVS PPDQAFFVLH KKSSDFVSFE CKNLPGTYIG VKDNQLALVE ENDESCNNIM FKLSKM
Synonyms IL-33
Accession Number Q66H70
GeneID 361749
Summary
Source Escherichia coli.
Molecular Weight Approximately 17.4 kDa, a single non-glycosylated polypeptide chain containing 156 amino acids.
Biological Activity Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 106 IU/mg.
Appearance Sterile filtered white lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2 um filtered concentrated solution in 20 mM Tris, 300 mM NaCl, pH 8.5.
Endotoxin Less than 1 EU/ug of rRtIL-33 as determined by LAL method.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
References 1. Lingel A, Weiss TM, Niebuhr M, et al. 2009. Structure. 17:1398-410.2. Baekkevold ES, Roussigne M, Yamanaka T, et al. 2003. Am J Pathol. 163:69-79.3. Onda H, Kasuya H, Takakura K, et al. 1999. J Cereb Blood Flow Metab. 19:1279-88.4. Black RA, Kronheim SR, Merriam JE, et al. 1989. J Biol Chem. 264:5323-6.
SDS-PAGE SDS-PAGE of Recombinant Rat Interleukin-33
Safety Data Sheet (SDS) Download Click to download
Technical Data Sheet (TDS) Download Click to download

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